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. 2014 Dec 5;5(2):157–166. doi: 10.1534/g3.114.015578

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Copyright © 2015 Biedler et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Knockdown of myd88 and dah mRNA in 48 hr PBM ovaries determined by droplet digital PCR. (A) myd88 mRNA is reduced in line nanos_2.5 (n = 6) by 73% compared with nontransgenic mosquitoes (NT, n = 6). Values shown are the mean ratios of myd88 to reference cDNA concentrations (proteasome subunit beta type-2). P-value is given at top. (B) dah mRNA is reduced in line vgr_2_2 (n = 8) by 43% compared with nontransgenic mosquitoes (NT, n = 4). Values shown are the mean ratios of dah to reference cDNA concentrations (Ribosomal protein S4). P-value is given at top. (C) Semi-quantitative RT-PCR using cDNA from adult individuals, from nontransgenic (NT) lines, and several nanos/anti-myd88 transgenic lines. Samples: 1-5, nontransgenic individuals; 6-7, line P1.4; 8-9, line P1.5; 10-11, line 2.1; 12-14, line 2.5; 15-17, line 2.6.