Table 1. Genotypic and phenotypic information about isogenic lines (IL) and hybrid F1s used to develop F2 seed populations.
Parental Line or F1a | Genotypea | Segment/Markerb | Pericarp Colorc | Plant Heightd |
---|---|---|---|---|
A. ILSD1-2 (EM93-1) | SD1-2SD1-2sd7-1sd7-1sd12sd12 | Recipient | White (rcrc) | Semidwarf (sd1sd1, ~80 cm) |
B. ILsd1-2 | sd1-2sd1-2sd7-1sd7-1sd12sd12 | ~3000 kb | White (rcrc) | Tall (Sd1Sd1, ~100 cm) |
C. ILSD7-1 | SD1-2SD1-2SD7-1SD7-1sd12sd12 | ~2 kb | Red (RcRc) | Semidwarf (sd1sd1) |
D. ILSD12 | SD1-2SD1-2sd7-1sd7-1SD12SD12 | ~200 kb | White (rcrc) | Semidwarf (sd1sd1) |
F1_SD1-2 (A×B) | SD1-2sd1-2sd7-1sd7-1sd12sd12 | RM315/3602 | White (rcrc) | Tall (Sd1sd1, ~90 cm) |
F1_SD7-1 (A×C) | SD1-2SD1-2SD7-1sd7-1sd12sd12 | RID12 | Red (Rcrc) | Semidwarf (sd1sd1) |
F1_SD12 (A×D) | SD1-2SD1-2sd7-1sd7-1SD12sd12 | SD12m13 | White (rcrc) | Semidwarf (sd1sd1) |
F1_SD7-1SD12 (C×D) | SD1-2SD1-2SD7-1sd7-1SD12sd12 | RID12 and SD12m13 | Red (Rcrc) | Semidwarf (sd1sd1) |
EM93-1 was the recipient of single introgression segments from a line of “red” weedy rice in ILs for the SD1-2, SD7-1 or SD12 locus. Upper or lower case letters indicate dormancy-enhancing (SD) or -reducing (sd) alleles at the three loci.
Physical lengths of the introgression segments in kilobases or DNA markers selected to tag the loci.
Rc, red pericarp color gene, which belongs to the same locus as SD7-1, with the functional allele responsible for both red pigment and enhanced seed dormancy (Gu et al. 2011).
sd1, semidwarf1 gene located on the SD1-2-containing region, with the EM93-1−derived allele responsible for both reduced plant height and enhanced seed dormancy (Ye et al. 2013).