Table 3. Summary of genotypic and allelic frequencies for the SD7-1, SD1-2, or SD12 locus in F2 seed subpopulations or joined populations.
Experimenta | Subpopulation (Genotyped Seeds)b | Endosperm Genotypic Frequencyc | χ2 Value (Probability)c | Allelic/Gametic Frequencyd | |||||
---|---|---|---|---|---|---|---|---|---|
Fddd | FDdd | FDDd | FDDD | Fd | Fd’ | Fd’’ | |||
SD7-1 | |||||||||
Ex. #1 (720, 0 DAR, 6%) | G (43, 100%) | 0.256 | 0.256 | 0.256 | 0.232 | 0.07 (0.995) | 0.512ns | 0.512ns | 0.511ns |
Ex. #2 (810, 3 DAR, 22%) | G (176, 98%) | 0.256 | 0.216 | 0.307 | 0.222 | 3.68 (0.298) | 0.517ns | 0.563ns | 0.472ns |
Ex. #3 (631, 10 DAR, 77%) | G (481, 99%) | 0.262 | 0.247 | 0.249 | 0.241 | 0.44 (0.932) | 0.510ns | 0.511ns | 0.509ns |
NG (138, 93%) | 0.283 | 0.232 | 0.246 | 0.239 | 0.84 (0.839) | 0.522ns | 0.529ns | 0.514ns | |
G+NG (619) | 0.267 | 0.244 | 0.249 | 0.241 | 0.99 (0.804) | 0.513ns | 0.515ns | 0.511ns | |
SD1-2 | |||||||||
Ex. #1 (1091, 1 DAR, 25%) | G (250, 92%) | 0.376 | 0.296 | 0.200 | 0.128 | 35.4 (<0.0001) | 0.624*** | 0.576* | 0.672*** |
Ex. #2 (1309, 10 DAR, 55%) | G (674, 93%) | 0.261 | 0.236 | 0.242 | 0.261 | 0.09 (0.710) | 0.500ns | 0.497ns | 0.503ns |
Ex. #3 (1096, 1 DAR, 24%) | G (280, 100%) | 0.396 | 0.300 | 0.207 | 0.096 | 55.3 (<0.0001) | 0.650*** | 0.604*** | 0.696*** |
NG (801, 99%) | 0.205 | 0.236 | 0.257 | 0.302 | 16.1 (0.0011) | 0.451** | 0.462* | 0.441*** | |
G+NG (1081) | 0.254 | 0.253 | 0.244 | 0.249 | 0.26 (0.967) | 0.503ns | 0.499ns | 0.507ns | |
SD12 | |||||||||
Ex. #1 (1980, 7 DAR, 20%) | G (381, 98%) | 0.367 | 0.234 | 0.257 | 0.142 | 39.4 (<0.0001) | 0.613*** | 0.625*** | 0.601*** |
Ex. #2 (639, 10 DAR, 34%) | G (215, 99%) | 0.381 | 0.195 | 0.228 | 0.195 | 20.4 (0.0001) | 0.593* | 0.609** | 0.577* |
Ex. #3 (633, 14 DAR, 26%) | G (161, 99%) | 0.372 | 0.186 | 0.298 | 0.143 | 21.2 (<0.0001) | 0.615** | 0.671*** | 0.559ns |
NG (234, 50%) | 0.269 | 0.209 | 0.291 | 0.231 | 3.77 (0.286) | 0.519ns | 0.560ns | 0.479ns | |
G+NG (395) | 0.544ns | 0.588*** | 0.499ns | ||||||
Ex. #4 (a random sample of 484 seeds) | 0.287 | 0.231 | 0.285 | 0.196 | 11.3 (0.0101) | 0.545* | 0.572** | 0.519ns | |
SD7-1 & SD12 | |||||||||
Ex. #1 (1529, 14 DAR, 29%) | G (436, SD12) | 0.477 | 0.181 | 0.216 | 0.126 | 127 (<0.0001) | 0.675*** | 0.693*** | 0.661*** |
G (436, SD7-1) | 0.225 | 0.239 | 0.280 | 0.257 | 2.97 (0.396) | 0.484ns | 0.504ns | 0.463ns | |
Ex. #2 (1019, 35 DAR, 81%) | NG (121, SD12) | 0.149 | 0.231 | 0.165 | 0.454 | 29 (<0.0001) | 0.347*** | 0.314*** | 0.380** |
NG (121, SD7-1) | 0.314 | 0.256 | 0.198 | 0.231 | 3.46 (0.326) | 0.541ns | 0.512ns | 0.570ns |
Listed in the parentheses are the total number of seeds received the number of days of after-ripening (DAR) treatment before the germination test and the mean germination rate at the seventh day after imbibition.
Listed in the parentheses are the number and proportion of genotyped seeds from the germinated (G) or nongerminated (NG) subpopulation.
The letters D or d in subscripts represent dormancy-enhancing or -reducing alleles in endosperm genotypes, which were tested against the 0.25:0.25:0.25:0.25 expectation.
The overall allelic frequency (Fd) and the male (Fd’) and female (Fd’’) gamete frequencies for the d allele are defined in Table 2. The estimates for SD12 in the joined population (G+NG in SD12 Ex. #3) are means weighted by the germination rate 26%. The superscripts indicate that the difference of the estimate from 0.5 was not significant at P = 0.05 (ns) or significant at P < 0.05 (*), <0.01(**), or <0.001 (***). The underlined estimates indicate Fd’’>Fd’ for SD1-2 or Fd’>Fd’’ for SD12 at the significance level of P < 0.05.