Table 3. Summary of genotypic and allelic frequencies for the SD_7-1, SD_1-2, or SD₁₂ locus in F₂ seed subpopulations or joined populations.

Experimenta	Subpopulation (Genotyped Seeds)b	Endosperm Genotypic Frequencyc				χ2 Value (Probability)c	Allelic/Gametic Frequencyd
		Fddd	FDdd	FDDd	FDDD		Fd	Fd’	Fd’’
SD7-1
Ex. #1 (720, 0 DAR, 6%)	G (43, 100%)	0.256	0.256	0.256	0.232	0.07 (0.995)	0.512ns	0.512ns	0.511ns
Ex. #2 (810, 3 DAR, 22%)	G (176, 98%)	0.256	0.216	0.307	0.222	3.68 (0.298)	0.517ns	0.563ns	0.472ns
Ex. #3 (631, 10 DAR, 77%)	G (481, 99%)	0.262	0.247	0.249	0.241	0.44 (0.932)	0.510ns	0.511ns	0.509ns
	NG (138, 93%)	0.283	0.232	0.246	0.239	0.84 (0.839)	0.522ns	0.529ns	0.514ns
	G+NG (619)	0.267	0.244	0.249	0.241	0.99 (0.804)	0.513ns	0.515ns	0.511ns
SD1-2
Ex. #1 (1091, 1 DAR, 25%)	G (250, 92%)	0.376	0.296	0.200	0.128	35.4 (<0.0001)	0.624***	0.576*	0.672***
Ex. #2 (1309, 10 DAR, 55%)	G (674, 93%)	0.261	0.236	0.242	0.261	0.09 (0.710)	0.500ns	0.497ns	0.503ns
Ex. #3 (1096, 1 DAR, 24%)	G (280, 100%)	0.396	0.300	0.207	0.096	55.3 (<0.0001)	0.650***	0.604***	0.696***
	NG (801, 99%)	0.205	0.236	0.257	0.302	16.1 (0.0011)	0.451**	0.462*	0.441***
	G+NG (1081)	0.254	0.253	0.244	0.249	0.26 (0.967)	0.503ns	0.499ns	0.507ns
SD12
Ex. #1 (1980, 7 DAR, 20%)	G (381, 98%)	0.367	0.234	0.257	0.142	39.4 (<0.0001)	0.613***	0.625***	0.601***
Ex. #2 (639, 10 DAR, 34%)	G (215, 99%)	0.381	0.195	0.228	0.195	20.4 (0.0001)	0.593*	0.609**	0.577*
Ex. #3 (633, 14 DAR, 26%)	G (161, 99%)	0.372	0.186	0.298	0.143	21.2 (<0.0001)	0.615**	0.671***	0.559ns
	NG (234, 50%)	0.269	0.209	0.291	0.231	3.77 (0.286)	0.519ns	0.560ns	0.479ns
	G+NG (395)						0.544ns	0.588***	0.499ns
Ex. #4 (a random sample of 484 seeds)		0.287	0.231	0.285	0.196	11.3 (0.0101)	0.545*	0.572**	0.519ns
SD7-1 & SD12
Ex. #1 (1529, 14 DAR, 29%)	G (436, SD12)	0.477	0.181	0.216	0.126	127 (<0.0001)	0.675***	0.693***	0.661***
	G (436, SD7-1)	0.225	0.239	0.280	0.257	2.97 (0.396)	0.484ns	0.504ns	0.463ns
Ex. #2 (1019, 35 DAR, 81%)	NG (121, SD12)	0.149	0.231	0.165	0.454	29 (<0.0001)	0.347***	0.314***	0.380**
	NG (121, SD7-1)	0.314	0.256	0.198	0.231	3.46 (0.326)	0.541ns	0.512ns	0.570ns

^aListed in the parentheses are the total number of seeds received the number of days of after-ripening (DAR) treatment before the germination test and the mean germination rate at the seventh day after imbibition.

^bListed in the parentheses are the number and proportion of genotyped seeds from the germinated (G) or nongerminated (NG) subpopulation.

^cThe letters D or d in subscripts represent dormancy-enhancing or -reducing alleles in endosperm genotypes, which were tested against the 0.25:0.25:0.25:0.25 expectation.

^dThe overall allelic frequency (F_d) and the male (F_d’) and female (F_d’’) gamete frequencies for the d allele are defined in Table 2. The estimates for SD₁₂ in the joined population (G+NG in SD₁₂ Ex. ^#3) are means weighted by the germination rate 26%. The superscripts indicate that the difference of the estimate from 0.5 was not significant at P = 0.05 (^ns) or significant at P < 0.05 (*), <0.01(**), or <0.001 (***). The underlined estimates indicate F_d’’>F_d’ for SD_1-2 or F_d’>F_d’’ for SD₁₂ at the significance level of P < 0.05.