Table 1. Summary of BLAST results and identification of markers used in Astyanax-to-Danio syntenic analysis.
| GBS Markers to Danio Genomea | GBS Markers to Astyanax Genomeb | Astyanax Genome to Danio Genomec | GBS Markers to Astyanax Transcriptomed | Astyanax Transcriptome to Danio Transcriptomee | |
|---|---|---|---|---|---|
| Total no. of BLAST queries | 2235 | 2235 | 2088 | 2235 | 572 |
| BLAST result categories | |||||
| Single robust hit | 14 (0.6%) | 1838 (82.2%) | 255 (12.2%) | 508 (22.7%) | 110 (19.2%) |
| Top hit, with positional support | 0 (0.0%) | 173 (7.7%) | 92 (4.4%) | 15 (0.7%) | 120 (21.0%) |
| Top hit, without positional support | 10 (0.4%) | 71 (3.2%) | 138 (6.6%) | 60 (2.7%) | 61 (10.7%) |
| Not top hit, with positional support | 2 (<0.1%) | 6 (0.3%) | 108 (5.2%) | 2 (<0.1%) | 7 (1.2%) |
| Unresolved | 4 (0.2%) | 14 (0.6%) | 4 (0.2%) | 12 (0.5%) | 0 (0.0%) |
| No result | 2205 (98.7%) | 133 (6.0%) | 1491 (71.4%) | 1638 (73.3%) | 274 (47.9%) |
| Identified syntenic markers between Astyanax and Danio | 26 | N/A | 593 | N/A | 298 |
a
Results of 64-bp GBS markers BLASTed directly to the Danio rerio genome.
b
Results of 64-bp GBS markers BLASTed directly to the Astyanax genome draft assembly.
c
Results of ∼2-kb genomic intervals harboring 64-bp GBS markers BLASTed to the Danio rerio genome.
d
Results of 64-bp GBS markers BLASTed directly to the Astyanax predicted transcriptome.
e
Results of Astyanax transcripts harboring 64-bp GBS markers BLASTed to the Danio rerio transcriptome.