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. 2014 Aug 26;80(2):216–229. doi: 10.1111/tpj.12624

Figure 1.

Figure 1

Overexpression of AtMYB41 in Arabidopsis leads to the ectopic deposition of lamellar structures in the cell walls of leaf epidermal cells and a leaf polyester monomer composition dominated by suberin-type monomers.(a) Transmission electron micrograph of a Col-0 (wild-type) leaf epidermal cell showing the intact cuticle and primary cell wall. Cut, cuticle; PCW, primary cell wall Scale bar = 100 nm.(b) Transmission electron micrograph of a leaf epidermal cell of AtMYB41 OE-9 showing a lamellar structure abutting the primary cell wall and an intact cuticle on the outer surface of the cell wall. Cut, cuticle; PCW, primary cell wall; LS, lamellar structure. Scale bar = 100 nm.(c) Leaf polyester monomer composition of wild type (Col-0) and AtMYB41 OE-9 from 6-week-old plants. Inset: total amounts of polyester grouped as cutin- and suberin-type monomers. All data are presented in micrograms per square centimeter of leaf area as mean values with SD (n = 4). FA, fatty acid; ω-OH, ω-hydroxy fatty acid; DCA, dicarboxylic fatty acid; di-OH, 10(9),16-dihydroxy fatty acid; alcohol, primary fatty alcohol; PP, phenylpropanoid. This experiment was performed twice with similar results. *Significant differences (P ≤ 0.01) as determined by Student's t-tests or Satterthwaite t-tests.