Table 1.
Relative quantification of suberin, cuticle, and lignin biosynthetic and Casparian strip assembly gene expression in AtMYB41 OE-9 leaves. Data are presented as mean fold change values ± SEM from three biological replicates. Fold change was determined by the comparative CT (ΔΔCT) method. Fold change is defined as normalized gene transcript levels from AtMYB41 OE-9 mRNA relative to normalized gene transcript levels in wild-type (WT) samples. Target gene transcript levels were normalized to the transcript levels of the endogenous controls GAPC (At3g04120), EIF4A1 (At3g13920), and UBIQUITIN5 (At3g62250). The induction of suberin biosynthetic genes was verified by semi-quantitative RT-PCR analysis (Figure S11)
| Gene | AGI | Fold change relative to WT |
|---|---|---|
| AtMYB41 | At4g28110 | 743 ± 79 |
| Suberin biosynthetic genes | ||
| FAR1 | At5g22500 | 10 ± 2 |
| FAR4 | At3g44540 | 1762 ± 141 |
| FAR5 | At3g44550 | 399 ± 76 |
| GPAT5 | At3g11430 | 8230 ± 826 |
| ASFT | At5g41040 | 3207 ± 561 |
| KCS2/DAISY | At1g04220 | 167 ± 39 |
| FACT | At5g63560 | 2226 ± 697 |
| CYP86B1/RALPH | At5g23190 | 7224 ± 869 |
| CYP86A1/HORST | At5g58860 | 18 603 ± 4950 |
| Cuticle biosynthetic genes | ||
| CYP86A2/ATT1 | At4g00360 | 0.8 ± 0.1 |
| CER1 | At1g02205 | 0.6 ± 0.2 |
| FAR3/CER4 | At4g33790 | 0.1 ± 0.1 |
| KCS6/CER6 | At1g68530 | 1.1 ± 0.5 |
| HOTHEAD | At1g72970 | 0.2 ± 0.1 |
| GPAT4 | At1g01610 | 4.5 ± 1.7 |
| GPAT8 | At4g00400 | 0.4 ± 0.2 |
| Phenylpropanoid and lignin biosynthetic genes | ||
| PAL1 | At2g37040 | 8.6 ± 1.3 |
| CCoAMT1 | At4g34050 | 3.8 ± 0.7 |
| CAD5 | At4g34230 | 36.1 ± 7.7 |
| C4H/REF3 | At2g30490 | 5.3 ± 1.7 |
| PRXR9/PER30 | At3g21770 | 27.5 ± 6.8 |
| Casparian strip assembly genes | ||
| CASP1 | At2g36100 | 2 ± 0.2 |
| ESB1 | At2g28670 | 1 ± 0.3 |