Figure 2. Kinase activity on FD T282 in protein extracts from shoot apices.

In-gel kinase assay with wild-type (WT) or T282A versions of C4 peptide (Fig. 1a) fused to GST or GST alone as a substrate. Extracts from shoot apices (SA) or whole aerial parts (Whole) of plants of indicated ages were analyzed. Kinase reactions were performed in the presence (+CaCl₂) or absence (+EGTA) of Ca²⁺.