Figure 8. G. mellonella larvae were injected by E. faecalis ×98 (purple), T2 CBS (orange), JH2-2 CBS (green), and a mixed suspension of either ×98 + T2 CBS (blue) or ×98 + JH2-2 CBS (red).

(A). Survival curves of G. mellonella larvae following infection with E. faecalis. Co-infection by T2 CBS and ×98 caused increased killing to that resulting by T2 CBS mono-infection, (P = 0.015, chi-square test with 1 degree of freedom, T2 CBS + ×98 versus T2 CBS). The plots display the averages of results of triplicate independent experiments. For each assay, 10 insects were used. (B). Real-time monitoring of CylL_S expression during the progression of an E. faecalis infection in intact insects. For tracking bacterial infection in G. mellonella, five individual insects were injected and placed in duplicate in a 4.0 cm Petri dish. Plates were kept at 37°C in the chamber of the Xenogen IVIS Lumina II imaging system (Caliper Life Sciences, CA). Bioluminescence was recorded at 30 minutes intervals for 20 hours.