Skip to main content
. 2014 Aug 7;79(6):1009–1019. doi: 10.1111/tpj.12602

Figure 5.

Figure 5

XCP2 induction by Ralstonia solanacearum is suppressed in the prn2-2 mutant. (a, b) Papain-like cysteine protease (PLCP) activity profiling with DCG-04 in protein extracts of leaves from WT (Col-0) and prn2-2 plants challenged by R. solanacearum. Active PLCPs are shown by streptavidin–HRP detection in the absence (a) or presence (b) of E64. (c) Western blot analysis with anti-XCP2 antibody (α-XCP2) of protein extracts of leaves from WT and prn2-2 plants challenged by R. solanacearum. (d) Western blot analysis with anti-RD21 antibody (α-RD21) of protein extracts of leaves from WT and prn2-2 plants challenged by R. solanacearum. (e) Input proteins are visualized by a Coomassie Brilliant Blue (CBB)-stained protein gel. T0, day 0 post-inoculation; DI1(−), unwilted leaves from DI1 plants; DI1(+), wilted leaves from DI1 plants. The assays were performed three times yielding similar results.