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. 2015 Jan 12;112(5):1475–1480. doi: 10.1073/pnas.1417731112

Fig. 5.

Fig. 5.

BPA-induced precocious neurogenesis is mediated via ARs and aromatase. (A) Quantification of neuronal birth in 5-dpf zebrafish coexposed from 0–5 dpf to 1 μM FAD + 0.0068 μM BPA and pulsed with EdU at 24 hpf. (B) Neuronal birth in 5-dpf zebrafish exposed from 0–5 dpf to 0.1 μM GSK4716 alone or coexposed to BPA + 50 nM amiodarone (AMIO) or to BPA + 6.17 μM flutamide (FLU). The red arrow indicates exposure at 8–48 hpf. The hash mark (#) indicates the AroB morphant (AroB-MO) exposed to BPA. (C) Neuronal birth in 5-dpf zebrafish exposed from 0–5 dpf to 0.0068 μM BPA or 1 μM DHT or coexposed to 1 μM DHT from 0–5 dpf and to 6.17 μM FLU (red arrow) for 8–48 hpf or to 1 μM ICI from 0–5 dpf. (D) Log-transformed relative AroB (cyp19a1b) expression at 48 hpf in zebrafish exposed to 0.0068 μM BPA or 1 μM DHT or coexposed to BPA or 1 μM DHT + 6.17 μM FLU or 1 μM ICI at 8–48 hpf. Data in A–D are shown as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (ANOVA, Tukey’s HSD); n = 3–13. (E) Diagram illustrating targets of various pharmacological agents and AroB MO.