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. 2015 Jan 20;112(5):1440–1445. doi: 10.1073/pnas.1424408112

Fig. 1.

Fig. 1.

Systemic and tissue-specific depletion of miR-8 results in decreased follicle size and dramatically reduced egg number. (A) Female mosquito ovaries at 24 h PBM. Ovaries were visualized using the Leica M165FC stereo microscope. (Scale bar: 1 mm.) (B) Average follicle size of miR-8Ant– and MsAnt-treated and noninjected mosquitoes at 24 h PBM. (C) Egg numbers per female mosquito for miR-8Ant– and MsAnt-treated and noninjected mosquitoes. (D) Mature miR-8 levels are decreased in Vg-Gal4/UAS–miR-8-SP (DA55/M3-2; DA55/M4-2) female mosquitoes as compared with controls. (E) Average follicle size of Vg-Gal4/UAS–miR-8-SP (DA55/M3-2; DA55/M4-2) miR-8–depleted females compared with Vg-Gal4/UAS–Scr-SP (DA55/M3-1; DA55/F5-1), Vg-Gal4 (DA55) driver, and WT controls 24 h PBM. Data represent averages. (F) Egg number per female mosquito for Vg-Gal4/UAS–miR-8-SP (DA55/M3-2; DA55/M4-2) females compared with Vg-Gal4/UAS–Scr-SP (DA55/M3-1; DA55/F5-1), Vg-Gal4 (DA55) driver, and WT controls. Data represent three biological replicates with three technical replicates and are shown as average ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001.