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. 2015 Jan 20;112(5):1440–1445. doi: 10.1073/pnas.1424408112

Fig. 2.

Fig. 2.

SWIM (AAEL001232) is a direct target of miR-8. (A) Putative miR-8 binding site within the AAEL001232 and AGAP007684 3′ UTRs. (B) Dual luciferase reporter assay for AAEL001232 (Swim). Data represent the percent activity (∆ fold activity × 100). Percentages are shown as the average ± SEM of triplicate samples. (C) AAEL001232 expression increases in Vg-Gal4/UAS–miR-8-SP (DA55/M4-2) female mosquitoes compared with controls (WT, DA55, and DA55/M3-1). (D) Long-range Wg signaling is reduced in Vg-Gal4/UAS–miR-8-SP (DA55/M4-2) female mosquitoes compared with controls (WT, DA55, and DA55/M3-1). (E) Short-range Wg signaling is unaffected in Vg-Gal4/UAS–miR-8-SP (DA55/M4-2) female mosquitoes compared with controls (WT, DA55, and DA55/M3-1). Data represent three biological replicates with three technical replicates and are shown as average ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001.