Fig. 5.

RIPA-insoluble proteins are stabilized in Ngly1^−/− MEF cells. (A) Cycloheximide chase assay of RIPA soluble/insoluble RTAΔm in WT, Ngly1^−/−, and Engase^−/− MEF cells. Cells expressing RTAΔm were chased using cycloheximide for the indicated time intervals. The RIPA soluble (lanes 1–3) and RIPA-insoluble extracts (lanes 4–6) were assessed. For each sample, extracts equivalent to 5 × 10⁴ cells were loaded. (B) Time course analysis of g1 and g0 levels in WT, Ngly1^−/−, and Engase^−/− MEF cells. Data represent average ± SD of three independent samples.