Fig. 7.

VH replacement of V_HQ52^NT employs adjacent V_H donor genes. (A) Schematic view of the D_H-proximal portion of the IgH locus mapping upstream of the V_HQ52^NT rearrangement. Colored blocks indicate functional germ-line V_H genes ordered based on their physical distance from V_HQ52^NT. Numbers above blocks indicate unique VH replacements using the indicated V_H genes. (B) qRT-PCR determination of V_H GLTs for the indicated V_H families in pro–B-cell cultures established from Rag1-mutant and V_HQ52^NT-HO mice. Levels of GLTs are relative to those of the housekeeping gene Rplp0.