Table 1.
Comparison of antibiotic resistance determinants found in the genomes of PA30 and PA49. Identifiers state PAO1 gene IDs or RefSeq Accession where applicable. Genotypes refer to presence or absence or genes or specific alleles with ‘wt’ indicating the genotype found in the reference strains PAO1
| Gene ID/accession | Gene name | Resistance type | PA30 genotype | PA49 genotype | Affected antibioticsa |
|---|---|---|---|---|---|
| gi|32470063 | aac(6’)-Ib | AAC(6’) | – | Present | Aminoglycosides (GM, AN) |
| gi|378773997 | aadB | ANT(2'’) | – | Present | Aminoglycosides (GM, AN) |
| gi|489251134 | blaVIM-2 | VIM | – | Present | Beta-lactams (AZ, CAZ, PT) |
| gi|88853419 | aadA10 | ANT(3'’) | – | Present | Aminoglycosides |
| gi|489211498 | – | AmpC | – | Present | Beta-lactams (AZ, CAZ, PT) |
| gi|489217979 | – | Metallo-beta-lactamase | – | Present | Beta-lactams (AZ, CAZ, PT) |
| gi|407937916 | – | Metallo-beta-lactamase | Present | – | Beta-lactams (AZ, CAZ, PT) |
| PA3168 | gyrA | Target modification | wt | T83I | Fluoroquinolones (CIP) |
| PA4964 | parC | Target modification | wt | S87L | Fluoroquinolones (CIP) |
| PA0958 | oprD | Decreased permeability | Multiple SNPs | Multiple SNPs, frameshift | Carbapenems (IPM) |
| insertion CC at position 279 |
a
Abbreviations indicate specific antibiotics. AN, amikacin; PT, Piperacillin + Tazobactam.
AZ, azlocillin; CAZ, ceftazidime; CIP, ciprofloxacin; GM, gentamicin; IPM, imipenem.