Figure 4.

DUX4 directly binds to FRG1 Peak1. (A) In vitro translated DUX4 protein binds to the labelled FRG1 Peak1 probe containing the TAATTCAATCA core sequence. Competition with unlabelled FRG1 Peak1 probe abolishes DUX4 binding, while competition with an excess of unlabelled FRG1 Peak1-mutated region probe (mutated core sequence TACTTCTATGA) does not. (B) DUX4 incubation with TRIM48 Peak region probe produces a shifted band that indicates direct DNA–protein binding, while FRG1 Peak2 region probe, containing a partial DUX4 core motif (TAATGTA), is not bound by DUX4. One representative experiment out of three independent experiments is shown.