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. 2014 Dec 5;24(5):1441–1456. doi: 10.1093/hmg/ddu561

Figure 2.

Figure 2.

DNA methylation of the HES4 promoter of HD and control cortex. DNA methylation states of a 269-bp fragment of the HES4 promoter in the PFC of 27 controls and 25 HD were measured by Methyl-Profiler as described in the Materials and Methods section. (Left) Examples of qPCR curves of all four reactions in one control and in one HD for the HES4 gene. DNA methylation states for the HES4 promoter are expressed as fractions of unmethylated (UM), intermediate-methylated (IM) or fully methylated (FM) DNA. (Right) IM was robustly increased from 5% of total input DNA in control to 49% in HD while UM fraction in the HES4 promoter was reduced in HD. In contrast, FM of the HES4 gene did not exhibit significant change.