| Method |
Vapour diffusion: sitting/hanging-drop method |
| Plate type |
Linbro (24-well) |
| Temperature (K) |
293 |
| Protein concentration (mgml1) |
14 |
| Buffer composition of protein solution |
20mM TrisHCl pH 8.0, 100mM NaCl, 5% glycerol, 1mM EDTA, 1mM DTT |
| Composition of reservoir solution |
0.1M bicine/Trizma base pH 8.7, 10%(w/v) PEG 20 000, 20%(v/v) PEG MME 550, 0.03M sodium nitrate, 0.03M disodium hydrogen phosphate, 0.03M ammonium sulfate |
| Volume and ratio of drop |
5l; 2:2:1 ratio of protein, reservoir solution and seeding stock |
| Volume of reservoir (ml) |
0.5 |
