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. 2014 Nov 4;66(3):813–825. doi: 10.1093/jxb/eru442

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Inhibition by ABA of H⁺ efflux from roots of Arabidopsis wild type (Col-0) and sextuple knockout of ABA receptors 112458 pyr/pyl (pyr/pyl). (A) Qualitative assay on plates with the pH indicator bromocresol purple. The result of a typical experiment is shown. A yellow colour around the roots after 8h indicates proton extrusion, and similar results were obtained in three independent experiments. (B) Quantitative determination of H⁺ efflux from plant roots. Sucrose (1%) was added to starved plants at time 0. Circles: wild type; squares: pyr/pyl mutant; closed symbols: control conditions; open symbols: 10 µM ABA added 10min before time 0. Values are means of three repetitions and standard errors (not shown for clarity) were 0.3–0.5 (Col-0, circles) and 0.6–1 (pyr/pyl, squares) nmol H⁺ mg^–1 of fresh weight (FW) of roots. (This figure is available in colour at JXB online.)