Fig. 3.

Cadaverine root length responses of chromosome 1 NILs and fine mapping. Relative root lengths on media containing 0, 100, and 500 μM cadaverine are shown. Plants were grown at a 30 ° backward tilt for 6 d. The average root length on control medium was set to 1. Absolute average root lengths on control medium were 1.0, 1.2, 1.2, 1.2, 1.1, 1.2, 1.1, and 1.1cm in the lines as shown from left to right. The asterisk indicates the first concentration on which the root length was significantly different from the length on control medium (P<0.05, Student’s t-test). Error bars indicate ±SE, and n=18–34. In the diagram of chromosome 1 NILs (bottom), white bars are Ler chromosomal regions, black bars are Cvi regions, and grey bars indicate regions in which the breakpoint is undetermined. The parallel dashed lines flank the probable region of a locus controlling root length responses to cadaverine. Accession breakpoints were determined using primers based on polymorphisms described by Monsanto/CEREON between Columbia and Ler (Jander et al., 2002).