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. 2015 Feb 9;6:68. doi: 10.3389/fmicb.2015.00068

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Copyright © 2015 Kistler and Broz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of peroxisomes and toxisomes in F. graminearum. Shown is a strain of F. graminearum having a GFP-tagged Pex3 protein and a TagRFP-T-tagged trichodiene oxygenase grown under trichothecene-inducing conditions. (A) Hypha visualized using differential interference contrast (DIC) microscopy. (B) TagRFP-T visualized by epifluorescence reveals the trichodiene oxygenase in spherical toxisomes in the subapical cells and in reticulate pattern toward the hyphal tip. (C) GFP fluorescence from Pex3 revealing puntate structures corresponding to peroxisomes. (D) Overlay of GFP and TagRFP-T fluorescence showing that peroxisomes are distinct from toxisomes. Bar = 10 μm. Results presented in Menke et al. (2013); figure generated for Weber (2013).