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. Author manuscript; available in PMC: 2015 Feb 9.
Published in final edited form as: J Neurosci Res. 2013 Mar 4;91(8):1066–1075. doi: 10.1002/jnr.23204

Figure 4.

Figure 4

OGD compared to hypoxia alone abrogates endogenous HIF-1α protein but not mRNA levels, and reduces cellular ATP levels. After 2 hour treatments with 10uM CPO, Hypoxia (1%O2, 20mM Glucose), or OGD (1%O2, 0mM Glucose): A. Representative immunoblot for HIF-1α; B. HIF-1α protein stabilization measured by densitometric analysis of three immunoblots; C. HIF-1α mRNA determined by qRT-PCR with beta-actin as endogenous control; D. ATP concentration, relative to untreated control. *p<.05, **p<.01, ***p<.001, n.s. no significant difference in ANOVA with post-hoc Tukey test.