OGD compared to hypoxia alone abrogates endogenous HIF-1α protein but not mRNA levels, and reduces cellular ATP levels. After 2 hour treatments with 10uM CPO, Hypoxia (1%O2, 20mM Glucose), or OGD (1%O2, 0mM Glucose): A. Representative immunoblot for HIF-1α; B. HIF-1α protein stabilization measured by densitometric analysis of three immunoblots; C. HIF-1α mRNA determined by qRT-PCR with beta-actin as endogenous control; D. ATP concentration, relative to untreated control. *p<.05, **p<.01, ***p<.001, n.s. no significant difference in ANOVA with post-hoc Tukey test.