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. Author manuscript; available in PMC: 2015 Feb 9.
Published in final edited form as: Stem Cells. 2014 Jun;32(6):1437–1450. doi: 10.1002/stem.1707

Figure 1. YB-1 over-expression in HMECs drove migration and luminal translocation in breast acinar structures.

Figure 1

(a) Immunoblot of YB-1 in the normal mammary epithelial MCF10A and 184hTERT cell lines compared to HTRZ and the YB-1-induced HTRY, HTRY-LT #1, and HTRY-LT #2 cell lines. (b) Immunoblot of YB-1 in HTRZ and HTRY cells compared to BLBC cell lines. (c) HTRY cells were grown as 3D acini on a reconstituted basement membrane. Following YB-1 induction, acinar structures were immunostained with ZO-1 (luminal), CD49f (basolateral), and CD44 antibodies. DAPI marked the nuclei. (d) Acinar structures (60 per time point) were evaluated for luminal filling and invasive outgrowths at the times indicated. (e) Acini were grown as above; however, YB-1 was induced along with the addition of DMSO or BI-D1870 (10 μM). At 96 hours luminal filling was evaluated. Scale bars represent 100 μm. UN, uninduced control. Data represented as mean ± SEM. P values were determined using t test. *, P <0.05; **, P < 0.01.