Figure 6. HTRY-LT cells were molecularly classified as BLBC.
(a) qRT-PCR and (b) immunoblot analysis of subtype biomarkers. MDA-MB-231 (TNBC), MCF-7 (ER+), and BT474 (PR+/HER2+) cells were used as controls. (c) qRT-PCR analysis of subtype biomarkers in HTRY-LT #2-derived xenografts from NSG mice. Data are reported relative to the appropriate control for each gene: MDA-MB-231 (EGFR+), T47D (ER+), and BT474 (ERBB2+ and PR+). (d) Box plot analyses of YB-1 expression among HU subtypes, Basal (n = 194), HER2 (n = 78), Luminal A (n = 221), Luminal B (n = 122), Normal-like (n = 121), and unclassified (n = 191). Data obtained using Gene expression based Outcome for Breast cancer Online (GOBO).