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. Author manuscript; available in PMC: 2015 Feb 9.
Published in final edited form as: J Immunol. 2009 Dec 30;184(3):1604–1616. doi: 10.4049/jimmunol.0903058

FIGURE 6.

FIGURE 6

CCR4+CCR6+, CCR4+ CCR6−, CXCR3+CCR6+, and CXCR3+ CCR6− T cell subsets exhibit differential cytokine/chemokine profiles. Memory CCR4+CCR6+, CCR4+CCR6−, CXCR3+ CCR6+, and CXCR3+CCR6− T cells were stimulated via CD3/CD28 for 3 d. A, Culture supernatants were screened for 60 soluble factors using the Cytokine Antibody Array VI (RayBiotec). Shown are results from one experiment representative of experiments performed with cells from two different donors. B, Levels of IL-10 (n = 11), TNF-α (n = 14), CCL20/MIP-3a (n = 14), CCL3/ MIP-1α (n = 6), and IL-2 (n = 12) were quantified by ELISA, whereas levels of CCL5/RANTES (n = 2) were quantified in cell supernatants by Cytometric Bead Array (mean ± SD). C, Shown are TNF-α/IL-10 ratios in CXCR3+CCR6+ and CXCR3+ CCR6− T cell subsets. Wilcoxon signed-rank test p values are indicated in the figure. Horizontal lines indicate median values.