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. Author manuscript; available in PMC: 2016 Jan 31.

Published in final edited form as: J Immunother. 2015 Feb-Mar;38(2):54–61. doi: 10.1097/CJI.0000000000000064

Tumor infiltrating lymphocytes were evaluated by flow cytometry on day 14 after B16GP33 tumor inoculation for levels of CD8+ and CD4+ T cells. CTLA-4 blockade and ACT resulted in the induction of higher levels of CD4+CD25+FoxP3+ T cells compared with control mice. (Representative individual data of CD4+CD25+ T cells shown in A, representative individual data of intracellular FoxP3 expression gated on CD4+CD25+ T cell populations shown in B.) (C) When represented as ratios of activated CD8+/CD44^high effector T cells to CD4+ regulatory T cells, a trend toward a favorable tumor infiltrating T cell profile was observed after combination immunotherapy (p=0.078 vs. control). This experiment (n=3-4 mice per group) was performed two times with similar results.

Tumor infiltrating lymphocytes were evaluated by flow cytometry on day 14 after B16GP33 tumor inoculation for levels of CD8+ and CD4+ T cells. CTLA-4 blockade and ACT resulted in the induction of higher levels of CD4+CD25+FoxP3+ T cells compared with control mice. (Representative individual data of CD4+CD25+ T cells shown in A, representative individual data of intracellular FoxP3 expression gated on CD4+CD25+ T cell populations shown in B.) (C) When represented as ratios of activated CD8+/CD44^high effector T cells to CD4+ regulatory T cells, a trend toward a favorable tumor infiltrating T cell profile was observed after combination immunotherapy (p=0.078 vs. control). This experiment (n=3-4 mice per group) was performed two times with similar results.