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. Author manuscript; available in PMC: 2015 Feb 9.
Published in final edited form as: Transfusion. 2012 Oct 9;53(7):1520–1530. doi: 10.1111/j.1537-2995.2012.03917.x

Fig. 1.

Fig. 1

SIPA in fresh and 4°C-stored PLTs. Washed PLTs (107/mL) were mixed with 5 μg/mL VWF and 2 mg/mL fibrinogen and subjected to shear rates of 0, 500, 2500, or 10,000/second for 120 seconds at 37°C, and PLT aggregation was measured. (A) Flow cytometric plots for the measurement of PLT aggregates. SIPA was estimated as increase in PLT population outside the single PLT gate compared to static controls. (B) Under static conditions, fresh or stored PLTs showed similar, baseline aggregation. (C) At different shear rates, the shear-induced aggregation of fresh or stored PLTs was normalized to the baseline static levels for each donor at the corresponding condition. Both fresh (□) and stored (▧) PLTs aggregate at high shear rates (n = 8, *p < 0.05, static vs. shear), but 4°C storage enhances aggregation (n = 8, §p < 0.05, ¶p < 0.01, fresh vs. stored). FSC = forward scatter; SSC = side scatter.