Fig. 3.

Effect of storage temperature on the binding of VWF under static (□) and shear (▧) conditions. Washed PLTs were mixed with 5 μg/mL VWF and subjected to static or shear (10,000/sec) for 120 seconds at 37°C. (A) The VWF binding was measured using a PE-conjugated anti-VWF polyclonal antibody. (B) The results are the mean of one experiment performed in triplicate, and the experiment was repeated for three donors. Shear stress increased the binding of VWF to PLTs over static incubation (n = 3, *p < 0.05, static vs. shear).