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. Author manuscript; available in PMC: 2015 Feb 9.
Published in final edited form as: Exp Neurol. 2013 Jan 9;247:359–372. doi: 10.1016/j.expneurol.2013.01.001

Figure 2.

Figure 2

Evaluation of the microinjection sites in the substantia nigra pars compacta (SNc). (A, B) Rat brains (n = 7) were stereotaxically injected on the left side with 0.1 μL of Evans blue to validate microinjection co-ordinates: −5.2 mm (AP) from Bregma, −2 mm (ML) from Bregma, −7.6 (V) mm from the dura. (C) A representative anatomical location of the microinjection sites in the SNc and its magnification. (D) Representative coronal sections from far ends of the SNc along the rostro-caudal axis (−4.48 to −6.12 mm with respect to Bregma) immunolabelled for parkin, demonstrating the highest parkin overexpression close to the injection site and the lowest at the ends of the SNc. Bars: 1000 μm. Abbreviations: AP, anterior-posterior; ML, medio-lateral; V, ventral.