Table 3.
Conc (ng/ml) |
PQ peak area CV (%) |
IS peak area CV (%) |
Peak area ratio CV (%) |
||||||
---|---|---|---|---|---|---|---|---|---|
Set 1 | Set 2 | Set 2–Set 1 | Set 1 | Set 2 | Set 2–Set 1 | Set 1 | Set 2 | Set 2–Set 1 | |
Low (3.0) | 5.2 | 1.6 | −3.6 | 7.5 | 4.5 | −3.0 | 4.3 | 3.9 | −0.4 |
Medium (20) | 5.3 | 2.5 | −2.8 | 5.9 | 2.9 | −3.0 | 3.2 | 4.1 | 0.9 |
High (200) | 4.8 | 3.5 | −1.3 | 5.5 | 2.2 | −3.4 | 1.1 | 4.1 | 3.1 |
Set 1 samples prepared in 5% trichloroacetic acid in MeOH-water(1:1, v/v), set 2 samples prepared in six different lots of plasma.
Conc: Concentration; CV: Coefficient of variation; IS: Internal standard; PQ: Piperaquine.