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. Author manuscript; available in PMC: 2015 Feb 9.
Published in final edited form as: Methods Mol Biol. 2013;1073:85–106. doi: 10.1007/978-1-62703-625-2_9

Figure 3.

Figure 3

(a) The vector map of pRS416m. (b) The vector map of the construct pRS416m-zeaxanthin. CEN6: centromere; ARS H4: automatic replication sequence; ura3: selection marker in S. cerevisiae; amp: selection marker in E. coli; hisG and delta2: two regions flanking the zeaxanthin biosynthetic pathway; PMB1: E. coli origin of replication. F1 ORI: this region is contained in the original pRS416 vector, but is not required for the construction of the plasmid containing the zeaxanthin biosynthetic pathway. (c) The vector map of the construct pZS-aur-2. oriR6K: E. coli origin of replication; accIV: apramycin resistance gene (it can be used as the selection marker in both Streptomyces and E. coli); oriT: conjugal transfer ori; PhiC31 attP: the ϕC31 recognition site; int: the ϕC31 integrase; tL3: a terminator.