Table 2.

The primers used in assembling the aureothin gene cluster variants. The mutated codons are underlined.

Name	Sequence
Aur-1-for	TGGTACTGCAAATACGGCATCAGTTACCGTGAGCAGATCGGATCAGCTCGTCCCGTTCGG
Aur-1-rev	GCTGCTCTTCTCGCATCGTC
Aur-2-for	CGTAGAGGAGCTCCAGCAGC
Aur-2-rev	CTCCTCCAGCACCTCGCAGC
Aur-3-for	TCCTGACCTTCGACTCGCTG
Aur-3-rev	CATGTTCGATCCTTCCGTTG
Aur-4-for	GACGGTGCACCAGCTGGTCA
Aur-4-rev	GTTGCCGGTCATGTGGTAGC
Aur-5-for	ATGACCAATGACGCCAAGAC
Aur-5-rev	CCGTCCATCAGGTCGAACGC
Aur-6-for	CCTACTACGGCCTGGTGGAC
Aur-6-rev	CATCGCCGTCATCGAGACGA
Aur-7-for	GCAACGAAGGACATGTCCAG
Aur-7-rev	TTATAGCACGTGATGAAAAGGACCCAGGTGGCACTTTTCGTCAGTCAGTCGTCCAGGCGC
Yeast-for	CGAAAAGTGCCACCTGGGTC
Yeast-rev	AATATTGTGAGTTTAGTATACATGCA
Strep-for	GTATTATAAGTAAATGCATGTATACTAAACTCACAATATT ATGGCGCGCCGACGTGCTCA
Strep-rev	ATTAGCCATGGCATCACAGTATCGTGATGACATTAATTAACGCAATCCAGTGCAAAGCTA
E. coli-for	AGACGAAGAAGCTAGCTTTGCACTGGATTGCGTTAATTAATGTCATCACGATACTGTGAT
E. coli-rev	GCCCATGACCACCGTCGTCTCCGAACGGGACGAGCTGATCCGATCTGCTCACGGTAACTG
AurB-DH-H964A-for	TGTCGGCGCGGACCGAGTCCTGGCTGGCCGACGCCGTCGTGCTCGGCTCCACGCTCGTCC
AurB-DH-H964A-rev	GGACGAGCGTGGAGCCGAGCACGACGGCGTCGGCCAGCCAGGACTCGGTCCGCGCCGACA
AurB-DH-D1131A-for	GCTACGGCGTCCACCCCGCGCTCCTCGCCGCCGCACTGCACACCGCCCTCCTGAAGGAGG
AurB-DH-D1131A-rev	CCTCCTTCAGGAGGGCGGTGTGCAGTGCGGCGGCGAGGAGCGCGGGGTGGACGCCGTAGC