Fig. 1.

Age-associated changes in NMNAT2-Venus axonal transport in sciatic nerve axons. (A) Representative straightened axon, kymograph, and kymograph of tracked particles of NMNAT2-Venus transport in sciatic nerves of 1.5- and 24-month-old NMNAT2-Venus (line 8) mice. The straightened axon represents the first frame of the time lapse recording (total 240 frames; frame rate 2 fps) that was used to generate the original kymograph. Moving particles were tracked using the ImageJ Difference Tracker set of plugins (see Table 2 for analysis parameters) and another kymograph generated to show successfully tracked particles. (B–E) Quantification of axonal transport parameters in sciatic nerve explants from NMNAT2-Venus line 8 animals of indicated ages. For all graphs, each data point represents the mean value obtained for 1 animal (5 fields of view and, on average, 14 axons per animal). Horizontal bar indicates mean, error bars are SEM. *Statistically significant difference between indicated ages or groups of ages. (*p < 0.05, **p < 0.01, ***p < 0.001, ***p < 0.0001; 1-way analysis of variance with Tukey multiple comparisons post-test or Student t test). The following parameters are shown: (B) anterograde particle velocity, (C) retrograde particle velocity, (D) anterograde particle count, and (E) retrograde particle count. (For interpretation of the references to color in this Figure, the reader is referred to the web version of this article.)