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Published in final edited form as: Gastroenterology. 1996 Dec;111(6):1714–1721. doi: 10.1016/s0016-5085(96)70037-9

Colonic explants release two peaks of biological activity that can be separated by HPLC. Activity was determined by applying an aliquot of each fraction to T84 cells. One unit is defined as the amount of material that increases the cGMP level in a well of confluent cells by 1 pmol/30 minutes. Arrowheads indicate the retention times of standards (1, guanylin 15; 2, uroguanylin; 3, STa; 4, proguanylin). The most prominent peak coelutes with the guanylin standard. Secretion of this material is significantly suppressed in rats from the low-salt group (*P < 0.04 compared with control). A second peak of unknown identity elutes in fraction 18. Secretion of this material is also suppressed in low-salt animals, although the statistical significance of the effect is slightly less (P = 0.059). ●, Low salt; ○, normal salt; , high salt.

Inline graphic — Colonic explants release two peaks of biological activity that can be separated by HPLC. Activity was determined by applying an aliquot of each fraction to T84 cells. One unit is defined as the amount of material that increases the cGMP level in a well of confluent cells by 1 pmol/30 minutes. Arrowheads indicate the retention times of standards (1, guanylin 15; 2, uroguanylin; 3, STa; 4, proguanylin). The most prominent peak coelutes with the guanylin standard. Secretion of this material is significantly suppressed in rats from the low-salt group (*P < 0.04 compared with control). A second peak of unknown identity elutes in fraction 18. Secretion of this material is also suppressed in low-salt animals, although the statistical significance of the effect is slightly less (P = 0.059). ●, Low salt; ○, normal salt; , high salt.