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. Author manuscript; available in PMC: 2015 Feb 10.
Published in final edited form as: J Invest Dermatol. 2008 Oct 30;129(2):422–431. doi: 10.1038/jid.2008.255

Figure 5. Over-expression of APE-1 rescued ROS-induced cell viability in MiTF-depleted cells.

Figure 5

(a) Concomitant knock-down of MiTF and over-expression of APE-1 in SK-MEL-28 cells. Cells were transiently transfected with pFlag-APE-1, and then infected with Ad-shLuc or Ad-shMiTF 24 hours later. (1) Control SKMel- 28 cells; (2) Cells transfected with pFlag-APE-1 and infected with Ad-shLuc; (3) Cells transfected with pFlag-APE-1 and infected with Ad-shMiTF. α-Tubulin serves as a loading control. (b) Cells with or without MiTF knock-down or APE-1 overexpression were treated with various concentration of glucose oxidase (Gox, left) or H2O2 (right) 48 hours post-infection, and MTT assay was performed to measure cell viability. All data is normalized to cells with Luc-ShRNA transfection.