FIG. 1.

Consideration of extracellular vesicles (EVs) in the design stage of tissue engineering approaches. Tissue engineers consider many parameters in designing therapeutic approaches, including cell source and culture conditions, biomaterial–cell interactions, and the site and method of therapeutic intervention (e.g., injection vs. implantation, use of an alternative anatomical location as an in vivo bioreactor). Consideration of each of these parameters could be affected by further knowledge of how EV biogenesis and cargo may change based on the choices made at each step of the tissue engineering design process. Color images available online at www.liebertpub.com/teb