Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 9;212(2):253–265. doi: 10.1084/jem.20141703

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Yang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — PDK1-mTOR signaling regulates IL-15–induced E4BP4 expression in vitro. (A) Quantitative RT-PCR analysis of NK cell–related genes in sorted wild-type CD3⁻NK1.1⁺ cells before and after stimulation with IL-15–IL-15R complexes; the results were normalized to those of β-actin and are presented relative to those of untreated cells, set as 1. Data were pooled from three independent experiments. **, P < 0.005; ***, P < 0.0005 versus control. (B) Intracellular staining was used to analyze the expression of E4BP4, Eomes, and T-bet on gated CD3⁻NK1.1⁺ cells by flow cytometry before and after stimulation with IL-15–IL-15R complexes; the results were normalized and are presented relative to MFI of untreated cells, set as 1. Data were pooled from four independent experiments. **, P < 0.005; ***, P < 0.0005 versus control. (C) Quantitative RT-PCR analysis of E4bp4 in sorted wild-type CD3⁻NK1.1⁺ cells after stimulation with IL-15–IL-15R complexes in the presence of the indicated inhibitors or of DMSO (control); the results were normalized to those of β-actin and are presented relative to those of DMSO-treated cells, set as 1. Data represent the mean ± SEM (3 repeats). **, P < 0.005 versus DMSO. (D) Intracellular staining was used to analyze the expression of E4BP4 and Eomes on gated CD3⁻NK1.1⁺ cells by flow cytometry after stimulation with IL-15–IL-15R complexes plus indicated inhibitors; the results were normalized and are presented relative to those of DMSO-treated cells, set as 1. Data represent the mean ± SEM (3 repeats). **, P < 0.005 versus DMSO. (E) Intracellular staining was used to analyze the expression of E4BP4 in CD3⁻CD122^high NK1.1⁺ NK cells by flow cytometry from indicated mice before and after stimulated with IL-15–IL-15R complexes. Representative overlaid histogram is shown (left). Red lines, unstimulated; Blue lines, stimulated. Results are presented relative to MFI values before treatment. Data were pooled from two independent experiments (right, n = 4). ***, P < 0.0005.