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. 2015 Feb 9;212(2):253–265. doi: 10.1084/jem.20141703

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© 2015 Yang et al.

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Figure 7. — PDK1 signaling is dispensable for NK cell terminal maturation and survival. (A) Representative flow cytometric profiles of NK1.1 versus NKp46 expression in splenic and BM CD3⁻ cells from the indicated mice. Numbers show the percentages in square boxes among gated CD3⁻ cells. (B and C) Flow cytometry analysis of NK1.1 versus CD122 expression in splenic CD3⁻ cells in Rosa26 DTA^STOP and Rosa26 DTA^STOP/Ncr1-Cre⁺mice (left). Expression of CD27 versus CD11b on CD3⁻NK1.1⁺CD122⁺ NK cells was further analyzed (right). The numbers indicate the percentages of cells in each quadrant. (C) Absolute number of splenic CD3⁻CD122⁺NK1.1⁺ cells (left) and four-stage differentiating NK cell subsets (left), including DN, CD27SP, DP, and CD11bSP, were quantified in Rosa26 DTA^STOP and Rosa26 DTA^STOP/Ncr1-Cre⁺ mice. n = 4. ***, P < 0.0005. (D) Flow cytometry analysis of NK1.1 versus CD122 expression in CD3-negative splenic and BM cells of PDK1^fl/fl and PDK1^fl/fl/Ncr1-Cre⁺ mice. Numbers show the percentages in indicated circles among gated CD3⁻ cells. (E) The absolute numbers of NK cells (CD3⁻NKp46⁺) in the spleens and BM of PDK1^fl/fl and PDK1^fl/fl/Ncr1-Cre⁺ mice were also quantified. Data were pooled from two independent experiments (n = 5–7). NS, not significant. (F) Representative flow cytometric profiles of the four-stage NK cell development, including CD27⁻CD11b⁻(DN), CD27⁺CD11b⁻(CD27SP), CD27⁺CD11b⁺(DP), and CD27⁻CD11b⁺(CD11b SP), from gated CD3⁻NK1.1⁺ NK cells in the spleens and BM of PDK1^fl/fl and PDK1^fl/fl/Ncr1-Cre⁺ mice are shown. The numbers indicate the percentages of cells in each quadrant.