Fig. 3.

(Left panels) Photomicrographs of NeuN (10×)-, glial fibrillary acidic protein (GFAP) (10×)-, and Iba1 (20×)-immunostained sections through the coronal section of striatum of control and 3-nitropropionic acid (3NP)-lesioned mice treated with vehicle or cannabigerol (CBG). They show a significant loss of NeuN-positive cells in the striatum of 3NP-treated mice compared with controls. CBG treatment significantly reduced 3NP-induced loss of striatal NeuN-positive cells. (Right panels) Quantification of (A) NeuN-, (B) GFAP-, and (C) Iba1-positive cells in the mouse striatum. Total average number of neurons, astrocytes (100× magnification) and microglia (200× magnification) is shown. Values are expressed as means ± SEM for 6–8 animals per group. Data were subjected to one-way analysis of variance followed by the Student–Newman–Keuls test. *P < 0.05, **P < 0.01, ***P < 0.001 when comparing the control group with the 3NP and CBG group. ^# P < 0.05, ^## P < 0.01, ^### P < 0.001 when comparing the 3NP group with the 3NP + CBG group