Fig. 5.

Paroxetine inhibits Ca²⁺-induced swelling in the brain mitochondria. Rat liver and brain mitochondria were de-energized by addition of 0.5 mM rotenone, 0.5 mM antimycin and 2 mM ionomycin. Mitochondria were then pretreated with fluoxetine (a) or paroxetine (b) for 10 minutes on ice and mitochondrial swelling was initiated by addition of 10 μM Ca²⁺. Mitochondrial swelling was monitored by measuring the decrease in optical density (OD) at 520 nm for 8 min post calcium addition. An analysis of variance showed that the effect of paroxetine on brain mitochondria was significant F[5, 13] = 15.37 , p < 0.0001). Post hoc analysis was done using Tukey’s multiple comparison test. Values represent mean ± SEM (3 experiments per condition, *p <0.05; **p < 0.01; ***p < 0.001)