Table 2.
MALDI/TOF-TOF analysis of selected protein spots from 2-Dimentional gel electrophoresis that differently expressed in sera from psoriatic and healthy human between two depletion methods
| Spot no*. | Protein name | MW (Da) | P-value | NCBI ID | TCA/aceton† | Aurum kit | ||
|---|---|---|---|---|---|---|---|---|
| H | P | H | P | |||||
| 1 | Unknown protein | 19941 | 6.47 | gi|119569581 | - | + | - | - |
| 2 | Keratin 10 | 39832 | 4.72 | gi|186629 | - | + | - | + |
| 3 | Chain A, The S Variant of human α1-Antitrypsin | 39099 | 5.27 | gi|231240 | - | + | - | + |
| 4 | Chain A, Crystal structure of cleaved antitrypsin | 37622 | 5.43 | gi|7546268 | - | + | - | + |
| 5 | Retinol binding protein 4 | 23371 | 5.76 | gi|18088326 | ++ | + | ++ | + |
| 6 | α1-AT (isoform 1) | 22871 | 6.11 | gi/28637 | - | - | - | + |
| 7 | α1-AT (isoform 2) | 22871 | 6.11 | gi/28637 | - | - | - | + |
| 8 | α1-AT (isoform 3) | 22871 | 6.11 | gi/28637 | - | - | - | + |
| 9 | α1-AT (isoform 4) | 22871 | 6.11 | gi/28637 | - | - | - | + |
α1-AT: Alpha1- antitrypsin; H: healthy; P: patients.
*
Spot no. related to the annotation in Figure 2.
†
+ existence; - absence; ++ increased concentration