Table 1.
Features of SNP, primer sequence, length of PCR product and restriction enzyme
| Name of SNP | Primer sequence | PCRproduct(b.p.) | Restriction enzyme |
|---|---|---|---|
| S1 (rs3918396) | Forward: 5′-TGTGCAGGCTGAAAGTATGC-3' | 304 | BtsCI |
| Reverse: 5′-AGAGCTCTGAGGAGGGGAAC-3' | |||
| T1 (rs2280091) | Forward: 5'-ACTCAAGGTGACTGGGTGCT-3' | 400 | NcoI (Bsp19I) |
| Reverse: 5'-GAGGGCATGAGGCTCACTTG-3 | |||
| T2 (rs2280090) | Forward: 5'-TTCTCAGGGTCTGGGAGAAA-3' | 310 | HpyCH4III (TaaI) |
| Reverse: 5'-GCCAACCTCCTGGACTCTTA-3' | |||
| V4 (rs2787094) | Forward: 5'-ACACACAGAATGGGGGAGAG-3' | 374 | PstI (BstMAI) |
| Reverse: 5'-CCAGAAGCAAAGGTCACACA-3' | |||