Table 1.
Horseradish peroxidase isoenzymes
| Isoenzyme | pI | MW kDa | GenBank | UniProt | References |
|---|---|---|---|---|---|
| C1A | 5.7 | 38.8 | M37156.1 | P00433 | (Fujiyama et al. 1988; Gajhede et al. 1997; Henriksen et al. 1998; Welinder 1976) |
| C1A | 5.7 | 38.8 | HE963800.1 | K7ZWW6 | (Näätsaari et al. 2014) |
| C1B | 5.7 | 38.6 | M37157.1 | P15232 | (Fujiyama et al. 1988) |
| C1B | 5.7 | 38.6 | HE963801.1 | K7ZW26 | (Näätsaari et al. 2014) |
| C1C | 6.2 | 36.5 | M60729.1 | P15233 | (Fujiyama et al. 1988) |
| 25148.1 (C1C) | 6.6 | 38.8 | HE963802.1 | K7ZWQ1 | (Näätsaari et al. 2014) |
| 25148.2 (C1D) | 7.0 | 38.8 | HE963803.1 | K7ZW56 | (Näätsaari et al. 2014) |
| C2 | 8.7 | 38.0 | D90115.1 | P17179 | (Fujiyama et al. 1990) |
| 04627 (C2) | 8.7 | 38.0 | HE963804.1 | K7ZW02 | (Näätsaari et al. 2014) |
| C3 | 7.5 | 38.2 | D90116.1 | P17180 | (Fujiyama et al. 1990) |
| C3 | 7.5 | 38.2 | HE963805.1 | K7ZWW7 | (Näätsaari et al. 2014) |
| A2 | 4.7 | 31.9 | --- | P80679 | (Nielsen et al. 2001) |
| A2A | 4.8 | 35.0 | HE963806.1 | K7ZW28 | (Näätsaari et al. 2014) |
| A2B | 4.8 | 35.1 | HE963807.1 | K7ZWQ2 | (Näätsaari et al. 2014) |
| E5 | 9.1 | 33.7 | – | P59121 | (Morita et al. 1991) |
| E5 | 8.7 | 37.9 | HE963808.1 | K7ZW57 | (Näätsaari et al. 2014) |
| N | 7.5 | 35.1 | X57564.1 | Q42517 | (Bartonek-Roxå et al. 1991) |
| 01805 | 6.4 | 39.1 | HE963809.1 | K7ZW05 | (Näätsaari et al. 2014) |
| 22684.1 | 6.8 | 37.7 | HE963810.1 | K7ZWW8 | (Näätsaari et al. 2014) |
| 22684.2 | 6.3 | 37.8 | HE963811.1 | K7ZW29 | (Näätsaari et al. 2014) |
| 01350 | 8.7 | 34.3 | HE963812.1 | K7ZWQ3 | (Näätsaari et al. 2014) |
| 02021 | 9.6 | 35.8 | HE963813.1 | K7ZW58 | (Näätsaari et al. 2014) |
| 23190 | 8.4 | 39.4 | HE963817.1 | K7ZWQ4 | (Näätsaari et al. 2014) |
| 04663 | 4.4 | 37.2 | HE963814.1 | K7ZW09 | (Näätsaari et al. 2014) |
| 06351 | 6.9 | 34.6 | HE963816.1 | K7ZW31 | (Näätsaari et al. 2014) |
| 03523 | 8.9 | 35.6 | HE963820.1 | K7ZWX0 | (Näätsaari et al. 2014) |
| 05508 | 8.6 | 34.3 | HE963815.1 | K7ZWW9 | (Näätsaari et al. 2014) |
| 22489.1 | 8.8 | 34.8 | HE963818.1 | K7ZW59 | (Näätsaari et al. 2014) |
| 22489.2 | 8.8 | 34.8 | HE963819.1 | K7ZW11 | (Näätsaari et al. 2014) |
| 06117 | 5.7 | 36.4 | HE963821.1 | K7ZW32 | (Näätsaari et al. 2014) |
| 17517.1 | 9.6 | 35.1 | HE963822.1 | K7ZWQ5 | (Näätsaari et al. 2014) |
| 17517.2 | 9.6 | 35.1 | HE963823.1 | K7ZW60 | (Näätsaari et al. 2014) |
| 08562.1 | 9.0 | 36.1 | HE963824.1 | K7ZW15 | (Näätsaari et al. 2014) |
| 08562.4 | 9.0 | 36.1 | HE963825.1 | K7ZWX1 | (Näätsaari et al. 2014) |
Natural HRP isoenzymes are shown with their corresponding GenBank and UniProt database accession numbers and references. Isoelectric points and molecular weights were predicted with the Compute pI/Mw tool (Bjellqvist et al. 1993, 1994; Gasteiger et al. 2005) using unprocessed amino acid sequences (if available; the isoenzymes A2, P80689, and E5, P59121, were only available as processed peptides). For isoenzyme C1A, the amino acid at position 37 is Ile according to the GenBank entry M37156.1 but Tyr according to the GenBank entry HE963800.1 and the UniProt entries P00433 and K7ZWW6; calculations of pI and MW were performed with the latter sequence