Figure 4.

ADSC-CM treatment induces apoptosis in T24 cell line. Cells were double-stained with annexin V and PI and analyzed by flow cytometry. The gate setting distinguished between living (bottom left), necrotic (top left), early apoptotic (bottom right), and late apoptotic (top right) cells. T24 cells were treated with DMEM (a) as a control. Apoptosis was induced by ADSC-CM treatment (b). The apoptosis of T24 cells (c) was increased in the test group more than the control group from three independent experiments (^** P < 0.01 versus control).