Figure 5. Effects of sirtinol on superoxide production and HNE activity in human neutrophils activated by stimulants other than fMLF.

Human neutrophils (6 × 10⁵ cells/mL) were incubated with DMSO (as control) or sirtinol (0.3–20 μM) for 5 min before being activated. Cells were activated with (A) PMA and (B) MMK1/CB, and superoxide release was measured. Cells were activated with (C) MMK1/CB and (D) PAF/CB, and HNE activity was measured. All data are expressed as mean ± S.E.M. (n = 3–7). ** p < 0.01; *** p < 0.001 compared with the control.