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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Anal Biochem. 2014 Nov 20;472:30–36. doi: 10.1016/j.ab.2014.11.008

Figure 4.

Figure 4

Isotope labeling efficiency of C. elegans proteins. Equal weights of the 13C6,15N2-lysine-labeled and unlabeled WT worms were pooled, and proteins were extracted by ultrasonication. The extracted proteins were then digested by Lys-C and analyzed by LC-MS/MS. The intensities of individual heavy-lysine labeled and unlabeled peptides are plotted against each other and linear regression analysis was performed. The observed intensities of labeled and unlabeled peptides were normalized with respect to TBB-1 (worm homolog of β-tubulin).