Figure 2. Mixing scheme to generate the SEQC RNA samples.

This figure was modified from Supplementary Figure S1 presented in the related research manuscript¹³. Samples MAQC-I–A and MAQC-I–B (top) were thawed from the stock acquired during the original MAQC-I study (2006) and aliquots then pooled (blue and grey tubes), adjusted to equal concentration, and then mixed with ERCC mix sets E and F (respectively). The ERCC mixes contain 4 subgroups of transcripts with different molar concentration ratios (4.0, 0.67, 0.5, and 1.0) defined between the two mixes (right bottom). Equal portions of these mixtures were then titrated in 3:1 and 1:3 ratios to create samples C and D (bottom). All four samples were finally separated into 10 μl aliquots for storage and distribution to the sequencing sites.