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. 2015 Feb 10;6:47. doi: 10.3389/fpls.2015.00047

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Copyright © 2015 Safiarian, Pertl-Obermeyer, Lughofer, Hude, Bertl and Obermeyer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional complementation assay and expression of LilKT1 in yeast K⁺ uptake mutant. (A) PLY240 mutants expressing HA-yLilKT1 could not grow at low K⁺ concentrations. Control experiments were performed with the wild-type strain PLY232 containing the empty expression vector pGREG535. (B) HA-tagged LilKT1 was detected in the membrane fraction of PLY 240 after induction with galacatose (+). The respective organelle membrane markers, Pma1p (plasma membrane H⁺ ATPase), Dpm1p (dolichol phosphate mannose synthase of ER) and the Vma1p subunit of the vacuolar V-type ATPase (V1-subunit) were detected in non-induced yeast cells. Arrowheads mark the expected molecular weights. Additional protein signals in the Pma1p lane are caused by secondary antibody. 40 μg protein per lane.