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On slide acetone fixation of Ectocarpus filaments. Filaments were fixed for 30 min in acetone without (A) and with (B) a prewash in water. Close-up of a burst A-type cell (C) and its schematic (D). General view of upright filaments (E). A, A-type; E, E-type; R, R-type. Arrow(s) indicate in (A) salt precipitate, in (B) branching cells, in (C), and (D) discharged cell content. Scale bar equals to 50 μm in (A,B), 25 μm in (C–E). The apical cell is depicted in yellow in (D).
